Resealed erythrocytes are gaining more popularity because of their ability to circulate throughout the body, biocompatibility, zero order release kinetics, reproducibility and ease of preparation. Control drug delivery system free download as powerpoint presentation. Propranolol, asparginase, methotrexate, insulin, metronidazole. Then, the cell suspension is incubated at 37 c to reanneal the resealed erythrocytes. Oct 27, 2011 resealed erythrocytes and monoclonal antibodies ppt. Ppt parenteral controlled drug delivery system powerpoint. Resealed erythrocytes as carrier for drugs provides secure and more effective drug targeting for longer period of time and have advantage over several drugs carrier models regarding the. Contents of the powerpoint on resealed erythrocytes include. Presentation bypresentation by sunilboreddysunilboreddy m.
Such cells could be used as circulating carriers to disseminate a drug within a prolonged period of time in circulation or in targetspecific organs, including the liver, spleen, and lymph nodes. Aug 27, 2009 the model of the erythrocyte membrane presented in cell biology, hematology, and biochemistry textbooks shows 2 major protein bridges that span between the phospholipid bilayer and the spectrinactin skeleton. Introduction basic concept of rbc drug carrying potential of rbc advantages and limitations source and isolation of. Erythrocytes, the most abundant cells in the human body, have potential carrier capabilities for the delivery of drugs. Such drugloaded carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from. Find erythrocytes stock images in hd and millions of other royalty free stock photos, illustrations and vectors in the shutterstock collection. Pdf resealed erythrocytes as a carrier for drug targeting. Biology chapter 5 osmosis and enzymes free download as powerpoint presentation.
Introduction definition and advantages formulation of sedds preparation of sedds mechanism of self emulsification in vitro evaluation of sedds improvement of oral absorption by sedds applications of sedds solid sedds recent approaches in sedds conclusion references download the powerpoint by liking us on. View antiamoebic drugs ppts online, safely and virus free. Erythrocytes are biocompatible, biodegradable, possess long circulation half lives and can be loaded with a variety of biologica. Introduction basic concept of rbc drug carrying potential of rbc advantages and limitations source and isolation of rbc.
Resealed erythrocytes as a carrier for drug targeting. Appropriate size, shape to permit passage through the capillaries. Ppt resealed erythrocytes powerpoint presentation free to. Resealed erythrocytes as drug carriers pharmatutor. Resealed erythrocyte has got several advantages over the other drug delivery system which makes it superior than other systems. Haematocrit value if blood is placed into a tube and centrifuged, the cells and the plasma will separate. The erythrocytes, which are heavy, will settle down to the bottom of the tube, while the plasma rises up to the top and the leukocytes.
Oct 02, 20 resealed erythrocytes properties as carrier. They protect the premature degradation, inactivation and excretion of proteins and enzymes they can target the drugs within reticuloendothelial system they facilitate incorporation of proteins and nucleic acids in eukaryotic cells by cell infusion with rbc. Contents introduction basic concept of rbc slideshow 2151823 by eli. Resealed erythrocyte red blood cell cell membrane free. The ability of resealed erythrocyte to deliver drug to the liver or spleen can be viewed as a disadvantage is that other organs and tissues are inaccessible. The erythrocytes, which are heavy, will settle down to the bottom of the tube, while the plasma rises up to the top and the leukocytes and platelets will form a thin layer buffy coat between the erythrocytes and the plasma. College of pharmacy, teerthanker mahaveer university, moradabad, uttar pradesh, india erythrocytes have been the most interesting carrier and have found to possess great potential in drug targeting.
Drugloaded carrier erythrocytes resealed erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from plasma, entrapping drug in. A free powerpoint ppt presentation displayed as a flash slide show on id. View antileishmanial drug ppts online, safely and virus free. The parameter indicates the effects of shear force and pressure by which resealed erythrocytes formulations are injected, on the integrity of the loaded cells. Resealed erythrocyte free download as powerpoint presentation. Hence, these carriers are called resealed erythrocytes. Nov 01, 2008 localization of glycolytic enzymes in murine erythrocytes resealed in the presence of the cytoplasmic domain of murine band 3 residues 98. Loaded rbc resealed loaded rbc loading buffer resealing buffer incubation at 25 0 c hypotonic preswelling 0. Loaded erythrocytes 10% haematocrit, 5 ml are passed through a 23gauge hypodermic needle at a flow rate of 10 mlmin. The major tyrosine kinase activity in erythrocytes appears membraneassociated, and direct phosphorylation can be observed in nonsealed ghost preparations after addition of mgatp.
They have a life span of 120 days and are reproduced erythropoiesis in the bone marrow under the regulation of erythropoietin. Scribd is the worlds largest social reading and publishing site. Seminar on resealed erythrocytes powerpoint ppt presentation. International journal of chemistry and pharmaceutical sciences. Most of the resealed erythrocytes used as drug carriers are rapidly taken up from blood by macrophages. Characterization of glycolytic enzyme interactions with.
Resealed erythrocytes are prepared by isolating erythrocytes from the plasma of the blood sample of an organism of interest, breakdown of cells using different methods and then entrapping the drug which is to be administered into the body into the erythrocytes by various techniques such as hypotonic haemolysis, hypotonic dilution, hypotonic. International journal of chemistry and pharmaceutical sciences 169 and serves only to carry haemoglobin. The assays of resealed membrane sialidases obtained from control and diabetic erythrocytes were routinely determined by fluorimetric methods. Jan, 2014 contents of the powerpoint on self emulsifying drug delivery systems sedds include. Erythrocytes erythrocytes are most abundant cells in human body 5. The erythrocyte integral proteins contain sialic acid which gives erythrocytes a negative charge. Murine wildtype cdb3 was dialyzed against 5 mm of potassium phosphate containing 160 mm of sodium chloride, ph 7. Resealed erythrocytes, crosslinking, cell recovery, endocytosis, slow release depot formulation.
Resealed erythrocytes are prepared by isolating erythrocytes from the plasma of the blood sample of an organism of interest, breakdown of cells using different methods and then entrapping the drug which is to be administered into the body into the erythrocytes by various techniques such as. Acidic and neutral sialidase in the erythrocyte membrane of. The use of cells as drug delivery system requires that the drug which are normally unable to permeate the membrane, should be made to traverse the membrane without causing any irreversible changes in membrane structure and. Resealed erythrocytes for drugloading carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from plasma, entrapping drug in the erythrocytes, and resealing the resultant cellular carriers5. Resealed erythrocytes as a carrier for drug targeting the pharma. Mar 20, 20 using this technique, drug present in the extracellular solution, it is possible to entrap up to 40 % of the drug inside the resealed erythrocyte and to use this system for targeted delivery via intravenous injection. Drug loading in bodys own erythrocytes when used to serve as controlled delivery system. Factors which considering resealed erythrocytes as carrier its shape and size to permit the passage through the capillaries. Aug 07, 2014 erythrocytes have been the most extensively investigated and found to posses great potential in novel drug delivery. Erythrocytes are the most abundant cells in the human body 5. Biology chapter 5 osmosis and enzymes cell membrane.
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